Shimadzu Spd20a User Manual Jun 2026
If you observe rising noise or ghost peaks, the cell may need cleaning or inspection.
Flush the cell with an appropriate solvent (e.g., Methanol or Nitric Acid for metallic contamination) at a low flow rate.
| Component | Frequency | Manual Section | Critical Warning | |-------------------|--------------------------------|----------------|------------------------------------------------------| | Flow cell | Weekly | 4.2 | Never touch the quartz lens with bare fingers. | | Deuterium lamp | Every 2000 hours or low energy | 4.3 | Allow lamp to cool 30 min before replacement. | | Cell gaskets | Annually or after 5000 hrs | 4.2.3 | Overtightening cracks the cell block. Torque to 2 Nm.| | Optical unit | As needed (dusty labs) | 4.4 | Only trained service engineers should open this. |
These specifications are critical for method development and ensuring your detector meets performance qualifications. shimadzu spd20a user manual
Open the control software (e.g., EZStart 7.4) and verify that the "remote" LED on the SPD-20A is lit, indicating proper communication.
If baseline noise increases or phantom peaks appear, the flow cell may need flushing with an appropriate solvent (e.g., methanol, water, or 1M nitric acid for specific contaminants). Lamp Replacement: The D2cap D sub 2
Turn the main power switch to ON. The power indicator bar on the right side of the display will turn green. If you observe rising noise or ghost peaks,
Flush the flow cell with a mobile phase that is transparent at your target wavelength.
Ensure the correct wavelength is selected and the flow cell is clean. Verify that the flow cell path is not obstructed.
This detector is certified as a Shimadzu Eco-Product, meaning it uses 35% less power than previous models without sacrificing its world-class noise level (0.5 x 10⁻⁵ AU). SR-Cell (Sensitivity and Resolution): | | Deuterium lamp | Every 2000 hours or low energy | 4
Press . The monochromator will physically shift, which you can hear as a brief motorized hum. 4. Routine Operation and Best Practices
The Shimadzu SPD-20A user manual provides critical, in-depth technical guidance for HPLC operators, covering installation, operation, and maintenance. It is available in both a brief instructional format and a detailed PDF version, featuring specific troubleshooting codes, safety protocols, and performance specifications for the UV-VIS detector. For access to the user manual, search for the document at Scribd .